Personal details - T.R. Nuringtyas
Sylvius Building, 3rd floor, Room 3.5.15.
Phone : +31 (0) 71 5275510
- Brief biography
2009 – today : PhD student
2001 – 2003 : MSc Molecular Biology and Biotechnology with thesis research : Molecular characterization of the ethylene receptor gene (ETR1) of carabao mango.University of the Philippines at Los Baňos, The Philippines.
1999 – today : Lecturer of Biochemistry at the Faculty of Biology, Gadjah Mada University, Indonesia.
1997 – 1999 : Research assistant at the Biochemistry Laboratory, Faculty of Biology, Gadjah Mada University, Indonesia
1991 – 1997 : Bachelor of Environmental Biology Faculty of Biology: Resistance of microorganisms to heavy metal contamination.Gadjah Mada University, Jogjakarta, Indonesia
The importance of variation of pyrrolizidine alkaloids in plant defense of Senecio
As a biochemist, I am especially interested in plant secondary metabolites as a result of interaction between plant with both biotic and abiotic environment. In my PhD project I am studying the origin and role of the diversity of pyrrolizidine alkaloids (PAs) and its importance for defense against generalist herbivores, such as the thrips Frankliniella oxidentalis and the caterpillar Spodoptera exigua. Diversification of PAs will be studied using metabolomics approaches, comparing different plant organs, tissues and cell types as. The possible role of endophytesrole in the diversification of PAs is also studied. The role of individual PAs and PAs in mixtures, to study potential synergistic effects, will be explored by using insect cell cultures.
- Student project
Why do different types of Pyrrolizidine Alkaloids (PAs) have different toxic effects ?
Info: Tri Rini Nuringtyas/Kirsten Leiss
Phone: 06 43771820/ 071 5275135
The wild plant Senecio is well known for its plant defence compounds the pyrrolizidine alkaloids (PAs). With these compounds the plant can defend itself against different insect herbivores such as caterpillars, locusts, aphids and thrips. At Plant Ecology and Phytochemistry we study the effect of different PAs on insects. Most approaches to study the effect of plant defence compounds on insects have used in vitro and in vivo insect bioassays. However, these tests are tedious, time consuming and expensive (Decombel et al., 2004). As an alternative we use insect cell lines to stud the toxicity of different PA forms and types on the Lepidopteran pest, beet armyworm (Spodoptera exigua). The study showed that different PAs vary in their toxicity.. The free base form of PA was more toxic compared to the N-oxide form. In terms of PA types, jacobine was the most toxic followed by erucifoline, senkirkine, seneciphylline and senecionine.
We now want to investigate why the different forms and types of PA show this varied toxicity. Insects are able to detoxify plant defence compounds by several enzymes which typically include three main super-families: the cytochrome P450 monooxygenases (P450s), the glutathione S-transferases (GSTs) and the carboxylesterases (COEs). In this proposed study we will use standard enzyme assays to measure the activity of these detoxification enzymes when the cell lines are treated with different forms and type of PAs. In this way we will get knowledge about the possible mode of action of the individual PAs.
Techniques : in vitro cell line assays, enzyme assays
Duration : 3-4 months